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. 2009 Mar 21;387(2):402–413. doi: 10.1016/j.virol.2009.02.021

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Copyright © 2009 Elsevier Inc. All rights reserved.

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Fig. 7 — The luminal domain of ATF6 is required for the association with 8ab. (A) Diagram of constructs encoding ATF6S1P⁻, ATF6ATF6 (475), ATF6 (430), ATF6 (418), and ATF6 (373). 3XFLAG was tagged at N-terminus of each protein. The transcriptional activation (TAD), basic leucine zipper (b-ZIP) and transmembrane domain (TM) are indicated. (B) ATF6 (418) or ATF6 (317) are not co-immunoprecipitated with 8ab. HeLa cells transiently transfected with the indicated plasmids were immunoprecipitated with anti-FLAG antibodies and immunoblotted with either anti-FLAG (top) or anti-HA antibodies (bottom). (C) Co-immunoprecipitation of ATF6 (475) and 8ab. HeLa cells transiently transfected with the indicated plasmids were immunoprecipitated with anti-FLAG antibodies and immunoblotted with either anti-FLAG (top) or anti-HA antibodies (bottom).