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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: J Biomed Mater Res A. 2019 Nov 26;108(3):601–613. doi: 10.1002/jbm.a.36840

FIGURE 4.

FIGURE 4

(a) Qrt-PCR analysis of CCND1, MDR1, Notch-1, and Survivin expression in MDA-MB-231 cells treated with LbL NPs carrying miR-34a or miR-co for 72 hr. GAPDH was used as a housekeeping gene, and mRNA expression is normalized to that of the miR-co LbL NP treated group. n = 3 or 4. (b) Western blot analysis of Bcl-2 and CCND1 protein expression in MDA-MB-231 cells exposed to LbL NPs carrying miR-co or miR-34a for 72 hr. The bands presented in this figure are cropped because the gels were used to probe for multiple proteins simultaneously. (c) Relative band density from Western blot images, as quantified using Image J and normalized to β-Actin, n = 3. Data were analyzed by Student’s t-test, *p < .05, **p < .01, ***p < .001, and ****p < .0001