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. Author manuscript; available in PMC: 2020 Jul 7.
Published in final edited form as: Int J Obes (Lond). 2020 Jan 7;44(4):948–963. doi: 10.1038/s41366-019-0512-z

Fig. 3.

Fig. 3.

AHR inhibition represses Scd1 expression and plasma arachidonic acid concentrations. (A) Total RNA isolated from liver was analyzed using microarrays from male B6 mice (n=4 mice per experimental group) fed control and Western diets with and without aNF (~90 mg/kg/day) or CH-223191 (~10 mg/kg/day) ad libitum for 5 weeks beginning at weaning and (B) from male B6 mice (n=3 mice per experimental group) fed control and Western diets with and without aNF (~90 mg/kg/day) ad libitum for 40 weeks beginning at weaning. (C) Proteins isolated from liver were analyzed by Western blotting from male B6 mice fed control and Western diets with and without aNF (~90 mg/kg/day) ad libitum for 40 weeks beginning at weaning. (D) Plasma arachidonic acid concentrations were determined by HPLC from male B6 mice (n=4 mice per experimental group) fed control and Western±aNF diets (aNF at ~90 mg/kg/day) ad libitum for 10 weeks beginning at weaning. (E) Total RNA isolated from liver was analyzed using microarrays from male B6 mice (n=3 mice per experimental group) fed control and Western diets with and without aNF (~90 mg/kg/day) ad libitum for 40 weeks beginning at weaning. C, Control diet; W, Western diet; WNF, Western+aNF diet; WCH, Western+CH-223191 diet. p-value: **, ≤0.01. Error bars represent SEM.