Figure 2.
Fluorescence emission spectra (λex: 280 nm) of 100 ng/mL OTA, OTB and OTC in MES buffer (pH 6.2, 10 mM) (A) and PB buffer (pH 8.6, 10 mM) (B). Fluorescence emission spectra (λex: 280 nm) of 5 μg/mL Nb28 and fluorescence excitation spectra (λem: 430 nm) of 100 ng/mL OTA, OTB, and OTC in MES buffer (C) and PB buffer (D). Both the MES buffer and PB buffer contain 0.5% Tween 20 and 5% methanol.