Fig 1.

Cell counts obtained by study enzymes at different time points. Kruskal–Wallis ANOVA showed a significant difference in immediate crude cell count for trypsin over different treatment times (p = 0.047). Post hoc test using Dunn’s test revealed that the significant differences were found between 15 min–24 h and 1h–24 h (p = 0.016). No significant difference was found for accutase (p = 0.52). Furthermore, a significant difference was observed for the 7 days group within the enzyme group (p = 0.007) but not for accutase (p = 0.066). Dunn’s test localized the differences to 15min–24 h, 1h–24 h, 5h–24 h (p = 0.03). No significant differences were found between trypsin and accutase at any of the time points. No significant difference was found between immediate crude cell count and crude cell count after 7 days of culture.