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. 2019 Mar 27;28(5):596–606. doi: 10.1177/0963689719825614

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 2. — Ki-67 staining of hRPCs as a marker of proliferation. (A–F) Representative photos of hRPCs cultured for 96 hours within Gtn-HPA (A–C) or on fibronectin (D–F). Ki-67 in green, DAPI in blue. Ki-67+ cells show clear heterochromatin-like pattern (bottom left). Ki-67+ signals are shown to have nuclear localization, from co-staining with DAPI. (G) Quantification of percentage population of Ki-67+ cells. Two-factor ANOVA did not show significant effect of culture conditions (Gtn-HPA vs. fibronectin) on percentage of Ki-67+ cell numbers after post hoc analysis (F(1, 12)=6.276, p=0.028; post hoc D1: p=0.213, D4: p=0.702, D7: p=0.467).