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. 2019 Mar 27;28(5):596–606. doi: 10.1177/0963689719825614

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 4. — Transplantation of GFP+ pRPCs in immunosuppressed Long Evans rats. Specimens were obtained 168 hours after transplant. (A–C) Examples of subretinally transplanted GFP+ cells with Gtn-HPA. Top of image = Vitreous side, Bottom = Choroidal side. GFP+ cells are below the outer nuclear layer (DAPI positive). (D) Example of subretinally transplanted GFP+ cells in saline control (HBSS-NAC). Looser distribution of GFP signal compared with A–C. No migration or integration into inner layers of retina seen with Gtn-HPA nor saline control. (E) Number of injected eyes with GFP+ findings, per 10 injections. Gtn-HPA showed more subretinally remaining transplants. A number of eyes showed GFP+ cells intravitreally in both groups, despite a confirmed subretinal bleb formation during transplantation. (F) Average transplantation grade of GFP+ eyes, calculated as average of five consecutive sections with the best grade (grade 3: >100 cells, grade 2: 10-100 cells, grade 1: 1–10 cells, grade 0: 0 cells per section). No significant difference seen between Gtn-HPA and HBSS-NAC groups.