Fig 2.

Expression profiles of the NIX protein after TBI. Western blotting was performed to analyze the expression of NIX in the peri-injury cortex at 6, 12, 24, 48, 72 h, and 7 d after TBI. Quantification graphs were produced by using NIX/GAPDH protein levels, and GAPDH was used as an internal standard to confirm equal quantities of protein loading. The results showed that the expression of NIX was decreased after TBI, and, at 24 h, the protein levels began to decrease significantly. Data are shown as the mean±SEM (n = 6, *P < 0.05, **P < 0.01 vs sham).