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. 2019 Apr 8;28(5):585–595. doi: 10.1177/0963689719840353

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig 4. — The changes in brain water content and neurological deficits after overexpression of NIX. a. Western blot and quantitative analysis were used to detect the efficiency of NIX overexpression at 24 h after TBI. b. Then, the brain water content was tested by the wet/dry method as well. The results were compared among each group not only in the ipsilateral hemisphere but also in the contralateral hemisphere. c. Behavioral tests were also carried out to define the specific effects of NIX. Quantitative analysis showed that neurological scores were obviously lower in the Over-NIX group. d. Albumin levels were significantly upregulated after TBI, and they decreased when NIX was overexpressed. Quantification graphs were generated for the results at the same time. Data are shown as the mean±SEM (n = 6, *P < 0.05 vs sham, ^#P < 0.05 vs Over-Ctr).