Table 1.
Type, Sources and Dilution of Antibodies.
| Antibody | Catalog number and company | Dilution |
|---|---|---|
| Primary antibodies | ||
| Polyclonal rabbit anti-PGP 9.5 | Z5116, Dako, Denmark | 1:200 |
| Polyclonal goat anti-NOS | sc-49055, Santa Cruz, USA | 1:100 |
| Monoclonal mouse anti-ChAT | sc-55557, Santa Cruz, USA | 1:100 |
| Monoclonal mouse anti-TH | AB318, Millipore, USA | 1:200 |
| Polyclonal rabbit anti-c-kit | A4502, Dako, Denmark | 1:100 |
| Monoclonal mouse anti-CD34 | M7165, Dako, Denmark | 1:100 |
| Polyclonal goat anti-PDGFR alpha | AF-307-NA, R&D Systems, USA | 1:100 |
| Monoclonal mouse anti-tryptase | M7052, Dako, Denmark | 1:100 |
| Secondary antibodies | ||
| Biotinylated goat anti-mouse | 115-065-146, Jackson ImmunoResearch, USA | 1:500 |
| Cy3-conjugated polyclonal goat anti-rabbit | 111-165-144, Jackson ImmunoResearch, USA | 1:500 |
| FITC-conjugated streptavidin | 016-010-084, Jackson ImmunoResearch, USA | 1:500 |
| FITC-conjugated polyclonal donkey anti-goat | J2609, Santa Cruz, USA | 1:40 |
| Alexa Fluor 488 Goat Anti-Mouse | 115-545-146, Jackson ImmunoResearch, USA | 1:400 |
| Alexa Fluor 594 Goat Anti-Rabbit | 111-585-144, Jackson ImmunoResearch, USA | 1:400 |
| Alexa Fluor 488 Rabbit Anti-Mouse | 315-545-045, Jackson ImmunoResearch, USA | 1:400 |
| Alexa Fluor 488 Goat Anti-Rabbit | 111-545-144, Jackson ImmunoResearch, USA | 1:400 |
| Alexa Fluor 594 Donkey Anti-Goat | 705-585-003, Jackson ImmunoResearch, USA | 1:400 |