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. 2019 Jun 4;25(6):374–388. doi: 10.1177/1753425919853333

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© The Author(s) 2019

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Proliferation analysis of T lymphocytes. (a) PBMCs from healthy donors (n = 3) were stained with CFSE before stimulation with 0.5 ng/ml LPS for 2 h and treatment with 0, 0.5 or 5 mg/ml trimodulin for 2 h. Subsequently, IL-2 (100 ng/ml) and/or ConA (10 µg/ml) were added to induce proliferation of T cells. (b) PBMCs from patients with early (n = 3) or late (n = 3) sepsis were treated only with trimodulin and ConA at the same concentrations as in (a). (c–e) After a total ex vivo incubation time of 72 h, (c) healthy donor PBMCs or PBMCs from patients with (d) early or (e) late-stage sepsis were analysed by flow cytometry to determine the percentage of CD3⁺ T lymphocytes that had divided up to seven times. Chi-square test, P<0.0001.