Table 4.
Trimodulin (mg/ml) | Phagocytosisa,b |
Oxidative bursta,c |
||||||
---|---|---|---|---|---|---|---|---|
24 h LTA+1 h trimodulin
(pHrodo-conjugated E.
coli) |
24 h LPS+1 h trimodulin
(FITC-labelled E. coli) |
Reactive oxidant-positive
monocytes |
Enzymatic activity |
|||||
LTA (µg/ml) | % of cells (n=3)d | LPS (ng/ml) | % of cells (n=7) | LPS (ng/ml) | % of cells (n=5) | Monocytes (n=5) | Granulocytes (n=5) | |
0 | 0 | 7.7 ± 2.1 | 0 | 92.0 ± 4.8 | 0 | 45.9 ± 14.6 | 2.3 ± 1.4 | 3.8 ± 1.5 |
0.5 | 0 | 7.5 ± 2.3 | 0 | 91.6 ± 5.4 | 0 | n.d. | n.d. | n.d. |
5 | 0 | 6.5 ± 3.0 | 0 | 90.8 ± 4.6 | 0 | 65.0 ± 11.6 | 3.1 ± 1.2 | 7.3 ± 1.7* |
0 | 1 | 7.0 ± 2.0 | 0.05 | 88.5 ± 6.2 | 0.05 | 56.2 ± 9.4 | 2.6 ± 1.3 | 5.3 ± 0.7 |
0.5 | 1 | 7.3 ± 2.2 | 0.05 | 89.8 ± 7.5 | 0.05 | n.d. | n.d. | n.d. |
5 | 1 | 6.6 ± 2.9 | 0.05 | 88.0 ± 7.4 | 0.05 | 60.2 ± 10.5 | 3.5 ± 1.1 | 6.6 ± 0.7 |
0 | 10 | 7.6 ± 1.2 | 0.5 | 91.0 ± 9.3 | 0.5 | 63.6 ± 12.4 | 2.8 ± 1.3 | 5.4 ± 1.1 |
0.5 | 10 | 7.7 ± 1.1 | 0.5 | 91.3 ± 9.5 | 0.5 | n.d. | n.d. | n.d. |
5 | 10 | 7.0 ± 1.8 | 0.5 | 91.6 ± 6.8 | 0.5 | 70.8 ± 10.6 | 3.8 ± 1.7 | 7.5 ± 2.1 |
Activities measured in whole blood samples.
Phagocytosis measured according to Figure 4a.
Oxidative burst after 24 h of LPS stimulation of whole blood and 1 h of trimodulin treatment (Figure 4c).
Mean and SD values obtained from a number (n) of donors as indicated.
*One condition comparison between untreated and trimodulin-treated cells resulted in P ≤ 0.05 (t-test). All data are mean (SD) unless otherwise specified.
n.d.: not determined.