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. 2019 May 17;25(6):344–355. doi: 10.1177/1753425919848841

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© The Author(s) 2019

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Phosphorylation of STAT1 (Y701) and NF-κB-p65 is decreased in macrophages due to HeLa, SiHa, and C-33A supernatants. Macrophages were obtained by differentiation of the U937 cell line with PMA (M0 macrophages), stimulation with LPS (M1 macrophages), cultured in the presence or not of 30% of HeLa, SiHa, or C-33A for 1 h (see Materials and methods), and harvested and processed by intracellular staining for measuring the expression of NF-κB-p65, STAT1(Y701), and STAT6 (Y641). Results are shown as % and MFI. Representative flow cytometric graphs of three independent experiments performed in triplicate are shown.