Figure 4.

Inheritance of genetic and epigenetic polycystic kidney disease (PKD)‐associated modifications. A, Variant allele frequency of somatic mutations observed in tubular epithelial cell (TEC) lines 6.1 and 9.1, also observed in induced pluripotent stem (iPS) cell lines 6A/B and 9A/B, respectively, are shown. B, Total number of uniquely called differentially methylated regions (DMRs, TSS, CpG island, and gene body) excluding overlapping regions. C, MeD‐seq profiles for the ZNF667 locus in wild‐type TEC, renal epithelium‐derived iPSC and embryonic stem cell lines. D, Unsupervised hierarchical clustering analysis of PKD and control iPSCs based on transcription start site DMRs observed between inter cell line comparisons. E, Overview of the number of DMRs observed in genome‐wide comparisons between PKD and control iPSC lines. F, MeD‐seq profiles showing a DMR observed between PKD and control iPSCs in an lncRNA gene. G, Gene Ontology (GO) analysis of genes hypermethylated DMRs in gene body region in PKD iPSCs