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. 2020 Mar 19;14:46. doi: 10.3389/fncel.2020.00046

Figure 2.

Figure 2

Pannexin 1 (Panx1) blockade does not affect neurodegeneration hallmarks in AD transgenic (Tg) mice. (A) Representative slot blot of soluble Aβ oligomers (sAβos) in hippocampal extracts immunodetected by A11 antibody (left panel). Relative levels of sAβos in 6-month-old (m.o.) wild-type (Wt) and Tg mice in the absence (Wt, black; Tg, red) or the presence of 100 μM probenecid (PBN; Wt+PBN, gray; Tg+PBN, blue; right panel). One-way ANOVA (F(3,11) = 6.640, p = 0.0146; N = 3) followed by Tukey’s post hoc test (p < 0.05). (B) Representative images of Kluver–Barrera- and Congo red-stained brain slices from 6 m.o. Tg mice in the absence (Tg, red) or the presence of 100 μM PBN (Tg+PBN, blue). Amyloid plaques are indicated by the arrows. Scale bar = 20 μm. (C) Number of amyloid plaques in dendritic layer (S rad.) of CA1 area of hippocampus (Hipp) and layer V (V) of the adjacent cortex (Cx). Unpaired, two-tailed t-test did not reveal significant differences (p = 0.6070 for number of plaques in Hipp, p = 0.9421 for number of plaques in Cx; N = 4 per group) vs. Tg. (D) Representative images of Nissl-stained brain slices from 6 m.o. Wt and Tg mice in the absence (Wt, black; Tg, red) or the presence of PBN (Wt+PBN, gray; Tg+PBN, blue). Pyknotic cells are indicated by the arrows. (E,F) Number of pyknotic and healthy neurons in pyramidal cell layer (S pyr.) of CA1 area of hippocampus (Hipp; E) and layer V (V) of the adjacent cortex (Cx; F). Two-way ANOVA (F(3,15) = 3.910, *p = 0.0302 for healthy cells in hippocampus; N = 3 per group) followed by Bonferroni’s post hoc test (p < 0.05) vs. Wt. (G) Representative images of GFAP immunofluorescence in brain slices from 6 m.o. Wt and Tg mice in the absence (Wt, black; Tg, red) or the presence of 100 μM PBN (Wt+PBN, gray; Tg+PBN, blue). (H) Astrocytic burden in dendritic layer (S rad.) of CA1 area of hippocampus (Hipp) and layer V (V) of the adjacent cortex (Cx). Scale bar: 200 μm. Two-way ANOVA (F(3,24) = 7.115, *p = 0.0014; N = 3 per group) followed by Bonferroni’s post hoc test (p < 0.05) vs. Wt. S pyr, stratum pyramidale; S rad, stratum radiatum.