FIGURE 6.

Western blot of Pop protein. Expression of pop in frame with a C-terminal SPA-tag. Cells were grown in LB at pH 7.4 and harvested before induction and up to 4 h after induction (t_x) and normalized cell numbers were separated on 16% tricine gels. The arrows indicate the band of the putative full-length protein Pop (bold arrow) and two shorter products (thin arrows). The experiment was conducted in LB at pH 7.4. L, Spectra Multicolor Low Range Protein Ladder (Thermo Scientific) with additional internal Western blot control glutathione-S-transferase (band sizes are indicated); u, whole cell extract from uninduced cells; t_x, whole cell extract from samples harvested after x = 0.5, 1, 1.5, 2, 2.5, 3, and 4 h after induction with arabinose.