Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jul 23;14(7):827–843. doi: 10.1586/14737159.2014.940319

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Informa UK, Ltd.

This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.

PMC Copyright notice

Figure 1. — Loop-mediated isothermal amplification. For clarity, only the process initiated in one strand is shown. (A) Primer design with inner primers (F1c-F2 and B1c-B2) and outer primers (F3 and R3). Loop primers are not shown. (B) Amplification process. (1) F2 region of the FIP primer anneals with DNA template by its homologous sequence (F2c) and strand extension take place by the polymerase. (2) DNA polymerase synthesizes a new strand from F3 and displaces the previous DNA strand. (3) F1c segment of F3 primer hybridizes with F1. At other end, B3 and BIP primers hybridize with their homologues sequences and process 1 and 2 are repeated. (4) Formation of loop DNA molecule. (5) DNA molecule steam loops on both ends enter in a cycling step and a concatemere final product with several inverted repeats is produced (6).

Image adapted from Eiken Genome site (http://loopamp.eiken.co.jp/e/lamp/principle.html).