Fig. 6.

Tier 2 confirmatory assay specificity determination. A. S/B values observed among drug naïve subjects (N = 31–37) with and without addition of 10 μg/mL of free drug. B. The percent reduction in S/B resulting from spiking with free drug was calculated for each sample. Gray bars and inset values indicate the threshold defining a specific or Tier 2 positive response, set at the 99th percentile of the observed distribution of degree of signal reduction among samples from drug naïve subjects for each bnAb.