Fig. 8.

Implementation of the tiered approach to defining ADA responses among NHP administered bnAbs and sampled longitudinally. A. Tier 1 ADA testing of serum samples from NHPs that received 3BNC117 (top, red) and/or 10–1074 (bottom, green). Individual animals are connected by a line and have the same symbol. Timepoints are colored in different shades. B. Tier 2 ADA testing at either the standard serum dilution factor (left) or a more dilute concentration of serum (right). C. Tier 3 testing of samples that were deemed specific in Tier 2. D. A summary of the number of animals that were Tier 1 or 2 positive broken out by whether the samples that were positive were from animals before (pre) or after (post) they received drug. The type of ADA (treatment-induced, treatment-boosted, or treatment-independent) are also identified for each animal deemed Tier 2 positive. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)