Figure 6.
RPB1 Stability Determines Transcription Recovery upon UV Irradiation
(A) Western blot showing the total levels of RPB1 before and after UV irradiation (10 J/m2).
(B) Diagram of cell lines.
(C) As in (A), but using the cell lines from (B) and testing later time points.
(D) RT-qPCR measuring nascent RNA production at the end of the long EXT1 and PUM1 genes at different times after different UV doses. Data are normalized to the expression of mature GAPDH transcript, and to untreated conditions, and represented as mean ± SD. Statistically significant differences (p < 0.05, multiple t tests, Holm-Sidak correction) in all three biological replicates are indicated with asterisks.
(E) Browser tracks from TTchem-seq experiments. The data are normalized to yeast spike-in.
(F) Metagene TTchem-seq profiles. Data are normalized to yeast spike-in.
See also Figure S6.