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. 2020 Mar 9;43(3):264–275. doi: 10.14348/molcells.2020.2197

Fig. 1. Expression levels of EGFR mRNA and protein are reduced in liver tissues of hepatocyte-specific eIF2α phosphorylation-deficient mice.

Fig. 1

(A) Quantitative real-time PCR analysis of the expression of Egfr mRNAs in liver tissues from 3-month-old Cont. and A/AHep mice. Data are expressed as mean ± SEM (n = 6 mice per group). ## P < 0.01; Cont. vs A/AHep. (B) Western blot analysis of eIF2α, p-eIF2α, EGFR, p-EGFR, and tubulin in liver tissues from 3-month-old Cont. and A/AHep mice. The efficiency of deletion of floxed eIF2α fTg by Cre recombinase in A/AHep livers was determined based on the existence of phosphorylated eIF2α proteins (Choi et al., 2017). (C) Densitometric quantification of EGFR and p-EGFR protein expression levels in Fig. 1B. Values were normalized against tubulin levels. Data are expressed as mean ± SEM (n = 4 mice per group). ### P < 0.001; Cont. vs A/AHep.