Table 5.

Differences between wild and vaccine strains of TW2575/98 at the nucleotide and deduced amino acid

No.	Nucleotide Substitution from TW2575/98w to TW2575/98vac	Deduced amino acid substitution	Switch of chemical characteristics of amino acid residue due to substitution	Specific passage which amino acid substitution was found	Gene involved
1	T147C	None			5′UTR
2	T684C	F53La	Np–Np	77b	p87
3	T737A	Silent
4	C976T	A150V	Np–Np	35	p87
5	A1380G	I285V	Np–Np	77	p87
6	C1388T	Silent
7	T1552A	V342D	Np → Ch	77	p87
8	T1588C	L354P	Np–Np	77	p87
9	T1616C	Silent
10	G3005A	Silent
11	T3013A	I829N	Np → Pu	19	HD1 (Ac domain)
12	G3021C	Silent
13	G3022C	G832P	Pu → Np	19	HD1 (Ac domain)
14	G3379A	G951E	Pu → Ch	35	HD1 (PL1pro)
15	G4365A	V1280I	Np–Np	35	HD1 (PL2pro)
16	T5004C	S1493P	Pu → Np	77	HD1
17	A5210G	Silent
18	T5445C	Silent
19	C5605T	A1693V	Np–Np	53	HD1
20	C6600T	P2025S	Np → Pu	69	HD1
21	T7450A	F2308Y	Np → Pu	69	HD1 (P41)
22	T9018G	W2831G	Np → Pu	53	3CLPro
23	A10137G	I3204V	Np–Np	53	HD2
24	G10565T	K3346N	Ch → Pu	53	HD2
25	G12025A	G3833D	Pu → Ch	35	GLF
26	C13645G	Silent
27	T13886G	F453V	Np–Np	19	RdRp
28	T13950C	L474P	Np–Np	53	RdRp
29	A14737T	Silent
30	T14971C	Silent
31	C16596T	P1356L	Np–Np	19	Helicase
32	C17332T	Silent
33	T18104C	Y1859H	Pu → Ch	19	ExoN
34	C20604A	P56T	Np → Pu	35	S1
35	G20718T	A94S	Np → Pu	35	S1
36	A22192G	E585G	Ch → Pu	35	S2
37	G22223T	L595F	Np–Np	69	S2
38	A22273T	N612I	Pu → Np	35	S2
39	C24535T	Silent
40	A24595T	E4V	Ch → Np	77	M
41	C24616T	S11F	Pu → Np	19	M
42	C25128T	R182C	Ch → Pu	35	M
43	T25641G	F7C	Np → Pu	35	5a
44	C27250T	None			3′UTR

Np nonpolar group, Ch charged group, Pu polar uncharged group

^aAmino acid changes in the specific residue

^bThe 77th passage of virus is vaccine strain