Figure 6.

IFNAR Signaling of Neurons and Astrocytes Regulates Activation of CNS Myeloid Cells

Animals were treated and brains were prepared on 6 dpi as described in Figure 1 for cryosectioning.

(A) OB slices were immunolabeled for Iba-1 and analyzed by confocal microscopy. Representative images of Iba-1⁺ myeloid cells from WT animals and from animals lacking IFNAR signaling on different CNS cell subsets.

(B) Quantification of numbers of primary processes, branching points, and process lengths of Iba-1⁺ myeloid cells (n = 10–28 cells from two to three animals per genotype, combined data). Data are shown as mean ± SEM. ^∗∗p < 0.005, ^∗∗∗p < 0.0005; ns, not significant. Scale bar in (A) is 10 μm.