Table 3.
Applications of next generation sequencing in medicinal plant research.
| S. no. | Plant | Biological uses | Platform used in NGS | Study | Reference |
|---|---|---|---|---|---|
| 1 | Ocimum sanctum | Used for cough, Cold, bronchitis, expectorant. | Illumina HiSeq2000 | Unraveling the genome of Holy basil: an “incomparable” “elixir of life” of traditional Indian medicine | Rastogi et al. (2015) |
| 2 | Beta vulgaris | For the treatment of fevers and constipation, disorders of the respiratory tract, fevers, and infections | Illumina HiSeq2000 | The genome of the recently domesticated crop plant sugar beet (Beta vulgaris) | Dhom et al. (2014) |
| 3 | Panax ginseng | To stimulate immune system, anticancer, and anti-hyperlipidemic | Illumina HiSeq | Transcriptome profiling and comparative analysis of Panax ginseng adventitious roots |
Jayakodi et al. (2014) |
| 4 | Elaeis guineensis | Used as laxative and diuretic, as a poison antidote, as a cure for gonorrhea, menorrhagia, and bronchitis | Roche/454 | “Oil palm genome sequence reveals divergence of interfertile species in Old and New worlds.” | Singh et al. (2013), |
| 5 | Curcuma longa | Anti-malarial, anti-inflammatory and antitumor | Illumina GAIIx | De Novo Transcriptome Assembly (NGS) of Curcuma longa L. Rhizome Reveals Novel Transcripts Related to Anticancer and Antimalarial Terpenoids |
Annadurai et al. (2013) |
| 6 | Catharanthus roseus | Anti-cancer | Illumina HiSeq2000 | CathaCyc, a Metabolic Pathway Database Built from Catharanthus roseus RNA-Seq Data | Van Moerkercke et al. (2013) |
| 7 | Withania somnifera | Restorative tonic, stress, nerve disorder, aphrodiasiac. | 454-GS FLX sequencing (454 Life Sciences, Roche, USA) | De Novo Assembly, Functional Annotation and Comparative Analysis of Withania somnifera Leaf and Root Transcriptomes to Identify Putative Genes Involved in the Withanolides Biosynthesis | Gupta et al. (2013) |
| 8 | Azadirachta indica | Sedative, analgesic, epilepsy, hypertensive. | Pyro-sequencing | A draft of the genome and four transcriptomes of a medicinal and pesticidal angiosperm Azadirachta indica | Krishnan et al. (2012) |
| 9 | Cannabis sativa | Hallucinogenic, hypnotic, sedative, analgesic, and anti-inflammatory agent | Illumina, Roche | “The draft genome and transcriptome of Cannabis sativa” | van Bakel et al. (2011) |
| 10 | Populus trichocarpa | Antipyretic, analgesic and to control inflammation | Expressed sequence tag based methods | “The genome of black cottonwood, Populus trichocarpa (Torr. & Gray)”. | Tuskan et al. (2006) |