Figure S2.

Effect of Translation and RNA Replication Inhibitors, Related to Figure 2

Wt MEFs were transfected with CVB3 replicon RNA (p53CB3-LUC), which contains the CVB3 cDNA in which the capsid-coding region is replaced by the firefly luciferase gene (Wessels et al., 2005), and incubated in the absence or presence of GuHCl (2mM) or CHX (10 μg/ml). Cells were collected every hour and the luciferase activity in the lysates was measured using a luminescent detector.