Figure S5.

Poly(I:C)-Induced IFN-β mRNA Upregulation in the Absence or Presence of CHX and DIP, Related to Figure 5

HeLa cells were transfected with 0.5 μg/ml poly(I:C) in the presence or absence of CHX (10 μg/ml) or DIP (100 μM). IFN-β mRNA levels were determined at 10 hr.p.t. by real-time qPCR. Both CHX and DIP treatments resulted in a reduced IFN-β mRNA induction in response to poly(I:C). Importantly, these results show that the difference in IFN-β induction we observed in CHX- and DIP-treated samples in Figures 5D and 5E could not have been artifacts caused by these inhibitors themselves. Data presented as mean ± SD.