Table 1.
Grouping of APP/PS1 mice for various lab and experimental procedures.
| Lab/behavioral investigations | Behavior evaluation (OF/NOR/MWM) | EEG recording | Nav expression analysis (Western blotting/cell surface biotinylation assay) |
||
|---|---|---|---|---|---|
| Grouping of APP/PS1 mice | Number of mice | ||||
| Early 12-week | Sedentary control | 2 m | 11 | 5 | 11 |
| 3.5 m | 11 | 5 | 11 | ||
| 5 m | 11 | 5 | 11 | ||
| Treadmill exercise | 2 m | 11 | 5 | 11 | |
| 3.5 m | 10∗ | 5 | 10∗ | ||
| 5 m | 9∗ | 5 | 9∗ | ||
|
| |||||
| Late 12-week | Sedentary control | 6.5 m | 10∗ | 4∗ | 10∗ |
| 8 m | 11 | 5 | 11 | ||
| 9.5 m | 11 | 5 | 11 | ||
| Treadmill exercise | 6.5 m | 10∗ | 5 | 10∗ | |
| 8 m | 10∗ | 5 | 10∗ | ||
| 9.5 m | 8∗ | 3∗ | 8∗ | ||
|
| |||||
| 24-week | Sedentary control | 2 m | 11 | 5 | 11 |
| 3.5 m | 11 | 5 | 11 | ||
| 5 m | 11 | 5 | 11 | ||
| Treadmill exercise | 2 m | 11 | 5 | 11 | |
| 3.5 m | 10∗ | 5 | 10∗ | ||
| 5 m | 9∗ | 4∗ | 9∗ | ||
∗Mice that died in this group were dropped out. The number of mice shows the actual number included in the analysis at the end of the experimental procedures; 2 m, 2-month-old; 3.5 m, 3.5-month-old; 5 m, 5-month-old; 6.5 m, 6.5-month-old; 8 m, 8-month-old; 9.5 m, 9.5-month-old. The age of the mice in the table corresponds to the age at the beginning of the study. OF: open field; NOR: novel object recognition task; MWM: Morris water maze test; EEG: electroencephalogram.