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. 2020 Mar 9;117(12):6890–6900. doi: 10.1073/pnas.1909943117

Fig. 5.

Fig. 5.

N-terminal glycosylation dependent cytoplasmic export of SIRT1. (A–D) Nucleo-cytoplasmic distribution of SIRT1 in HeLa cells treated with (A) 100 µM PUGNAc for 12 h and (B) 100 µM BZX for 12 h under HG conditions; (C) expressing either FLAG-SIRT1WT or -E2mut-AA under HG conditions, and (D) treated with 100 µM PUGNAc and 10 ng/mL LMB for 12 h. (E) Representative immunofluorescence image for endogenous SIRT1 localization upon treatment (16 h) with either OGT inhibitor (BZX) or OGA inhibitor (PUGNAc) in HeLa cells grown in LG or HG media, as indicated (n = 3 to 4). (F) Schematic representation of cytosolic export of SIRT1 upon nutrient dependent glycosylation at the N terminus. C, cytoplasmic fraction; N, nuclear fraction; T, total cell lysate.