Figure 3.

HPIV3 IBs Are Enriched with PI4P

(A) HeLa cells were transfected with N-Myc (0.1 μg) and HA-P (0.4 μg) for 24 h and then analyzed to detect PI4P on IBs. The fluorescence intensity profile of IBs (green) and PI4P (red) was measured along the line drawn on a 2× zoom panel by Leica Application Suite Advanced Fluorescence Lite.

(B) HeLa cells stably expressing GFP-P were infected with HPIV3 (MOI = 0.1) for consecutive times (0 h, 12 h, 24 h, 30 h, and 36 h) and then analyzed to detect PI4P on IBs. Yellow arrows indicate representative colocalization of PI4P and IBs.

Scale bars, 10 μm. See also Figure S3.