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. 2019 Nov 19;29(8):2229–2242.e4. doi: 10.1016/j.celrep.2019.10.052

Figure 6.

Figure 6

PI4KB Is Recruited to IBs via Its Interaction with P

(A) HEK293T cells were transfected with empty vector (2 μg) and HA-P (1 μg) or with FLAG-PI4KB (2 μg) and HA-P (1 μg) for 36 h. Cell lysates were subjected to IP and analyzed via WB.

(B) HEK293T cells were transfected with empty vector (1 μg) and FLAG-PI4KB (2 μg) or with HA-P (1 μg) and FLAG-PI4KB (2 μg) for 36 h. Cell lysates were subjected to IP and analyzed via WB.

(C) HEK293T cells were transfected with HA-P (1 μg) or empty vector (1 μg) for 36 h. Cell lysates were subjected to IP and analyzed via WB.

(D) HEK293T cells were transfected with empty vector (2 μg) and N-Myc (1 μg) or with FLAG-PI4KB (2 μg) and N-Myc (1 μg) for 36 h. Cell lysates were subjected to IP and analyzed via WB.

(E) HEK293T cells were transfected with empty vector (1 μg) and FLAG-PI4KB (2 μg) or with N-Myc (1 μg) and FLAG-PI4KB (2 μg) for 36 h. Cell lysates were subjected to IP and analyzed via WB.

(F) HEK293T cells were transfected with empty vector (2 μg), N-Myc (1 μg), and HA-P (1 μg) or with FLAG-PI4KB (2 μg), N-Myc (1 μg), and HA-P (1 μg) for 36 h. Cell lysates were subjected to IP and analyzed via WB.

(G) HEK293T cells were transfected with empty vector (1 μg), HA-P (1 μg), and FLAG-PI4KB (2 μg); with N-Myc (1 μg), HA-P (1 μg), and FLAG-PI4KB (2 μg); or with NL478A-Myc (1 μg), HA-P (1 μg), and FLAG-PI4KB (2 μg) for 36 h. Cell lysates were subjected to IP and analyzed via WB.