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. 2020 Mar 25;15:2071–2083. doi: 10.2147/IJN.S232677

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© 2020 Zhao et al.

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Effect of adjuvant formulations on humoral immune responses induced by different adjuvants. (ICR mice were immunized with 1.8 μg inactivated split-virus influenza vaccine mixed with 200 μL adjuvant on days 0, 7, and 21 by intramuscular injection). Antigen-specific IgG (A), IgG1 (B) and IgG2a (C) were determined by indirect ELISA. The serum 35 days after fist immunization was added as a primary antibody to the empty plate (has been coated with the capture antigen, and HRP-labeled human anti-mouse IgG, IgG1, IgG2a as secondary antibodies). The results were shown as the arithmetic mean, n = 5 per group.