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. 2020 Mar 10;117(12):6559–6570. doi: 10.1073/pnas.1919698117

Fig. 6.

Fig. 6.

The interaction with SNAP-25 is required for the plasma localization of SCGN. (A) Co-IP analyses of SNAP-25 knockout STC-1 clones stably expressing N-terminal HA tagged SNAP-25 (wild-type, G155D, R161H) or empty vector. Cell lysates were precipitated with anti-HA affinity matrix and probed for endogenous SCGN with anti-SCGN antibody. (B) Parental, SNAP-25 KO and SNAP-25 KO rescue STC-1 cells were costained with anti-SCGN and anti–SNAP-25 antibodies. (Scale bars, 10 µm.) (C) Quantification of SCGN membranous and total cellular fluorescence intensity ratio was calculated from each of the cell lines used in B. On average ∼40 cells were used for analysis in each group. Bars, mean; error bars, SEM; differences among groups by unpaired Student t test. ****P < 0.0001.