Fig. 3.

Simultaneous deletion of RAX1 and RSR1 results in random MPs in low αF. Distribution of the angle ω in daughter cells for the first bud (red) or the MP in response to uniform 5 nM α-factor (green) in the parent strain (ACL379) (A) or in the indicated deletion strains (B–H). In all cases, the angle distributions were divided into 30° bins, and the fraction of cells in each bin was calculated. Note that the data for strain ACL379 are the same as in Fig. 2A but displayed in a linear histogram (absolute value of ω). Data from at least three to four independent experiments were pooled to calculate the means ± SEMs (bars and whiskers). Strains: parent strain (ACL379); Δrsr1 (YGV5405); Δbud8 (YGV5429); Δbud8 Δbud9 Δaxl2 (YGV5433); Δrax1 (YGV5259); Δrax1Δxl2 (YGV5408); Δrsr1Δrax1 (YGV5838); and Δbud8 Δbud9 Δaxl2 Δrax1 (YGV5839).