Table 1.

Diversity and novelty of microorganisms isolated from wastewater treatment samples using different isolation procedures.

Culture conditions	Number of isolates	Number of phylotypesa	Shannon diversity index	Number of novel phylotypesb	Novelty indexc
Aerobic
PT	68	34	3.26	5	0.074
PS	67	40	3.09	13	0.194
Nitrate reduction
PT	41	19	2.55	5	0.122
PS	45	24	2.72	10	0.222
Fermentation
PT	35	6	1.62	1	0.029
PS	40	13	2.39	4	0.100

^a Isolates were assigned to phylotypes based on partial 16S rRNA gene sequences, with a sequence identify cut-off value of 97%.

^b Phylotypes with less than 80% classification reliability at the genus level in the RDP Classifier analysis were defined as novel.

^c The novelty index was calculated as “the number of novel phylotypes/total number of isolates”.