Fig. 3.

Respiration and H₂O₂ generation by mitochondria isolated from different tissues from adult mice (A–F), mouse embryos (G–I), and adult NMRs (J and K). Additions to the incubation mixture: 50 μM CArt, 3 mM creatine, 1 μg oligomycin (Oligo), 1 mM glucose, 10⁻⁷ M FCCP, 2 μM rotenone, and 0.5 mM KCN. Mitochondria were isolated in 300 mM mannitol, 0.5 mM EDTA, and 20 mM Hepes-KOH, pH 7.6. Mitochondria were incubated in medium containing 220 mM mannitol, 10 mM potassium lactobionate, 5 mM potassium phosphate, 2 mM MgCl₂, 10 μM EGTA, and 20 mM Hepes-KOH, pH 7.6. State 2 respiration was initiated by adding succinate (10 mM). State 3 or 3u was induced by the addition of 100 µM ADP or 10 nM FCCP, respectively. The concentration of mitochondria in the incubation vessel was 0.1 mg protein/mL. The temperature was 25 °C.