Fig. 8.

(A and B) Average values of glucose stimulation or the RCR for all tissues in mice and NMRs of various ages. Stimulation of State 4 respiration by glucose disappears with age in mice but not in NMRs. In mice, RCR decreases with age, but this effect is lower than that of glucose stimulation of respiration (A). In NMRs, the RCR does not depend on age (B). RCR was measured as the ratio of the State 3u respiration rate in the presence of uncoupler FCCP to State 4o (respiration rate after oligomycin addition). (C and D) Age-dependent oxidation of lipids and proteins in mouse and NMR tissues. The average values in all tissues of the malondialdehyde level (TBARS probe, lipid peroxidation) (C) and protein carbonylation level (D) are shown. Black, mice; red, NMRs. The data measured for individual tissues are shown in SI Appendix, Figs. S22 and S23.