Fig. 7.

UGT76F1 functions in regulating IAA amount. (A) DR5:GUS expression of indicated seedlings showing auxin responses. (Scale bars: 200 µm, Upper; 100 µm, Lower.) (B) IAA content measurement of indicated seedlings grown in continuous white light (70 µmol m⁻²⋅s⁻¹) conditions at 22 °C or 28 °C for 5 d. Data are means ± SD. n = 3. FW, fresh weight. Asterisks indicate significant differences relative to respective WT at 22 °C or 28 °C (Student’s t test, *P < 0.05). (C) Phenotypes of seedlings grown with or without the synthetic auxin picloram (PIC). Seedlings were grown in continuous light at 22 °C for 2 d and then transferred to plates containing picloram at 28 °C for another 2 d. (Scale bars, 1 mm.) (D) Hypocotyl length of seedlings shown in C. Data are means ± SD. n = 30. Significant difference was compared to WT (Student’s t test, *P < 0.05).