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. 2020 Mar 18;2020:2953068. doi: 10.1155/2020/2953068

Figure 2.

Figure 2

IL-4 treatment on donor livers suppressed apoptosis and inflammation induced by IRI. (a, b) Representative images of TUNEL-stained hepatocellular apoptosis in liver grafts at 6 h after liver transplantation (original magnification, ×200) and quantification of TUNEL-positive cells (n = 6/group). (c) The protein levels of Bcl2, Bax, and cleaved caspase3 at 6 h after liver transplantation were tested by Western blot. (d) The mRNA levels of proinflammatory factors (IL-1β, IL-6, and TNF-α) in the liver graft at 6 h after liver transplantation were detected by qRT-PCR. (e) The protein expression level of IL-1β, p-p65, and p65. GAPDH served as an internal control and was used for normalization. Data are shown as mean ± SD, ∗p < 0.05vs. the Sham group, #p < 0.05vs. the LT + IL-4 group.