Fig. 2.
Fluorescence assay monitoring peptoid-stabilized duplex DNA (dsDNA). Real-time SG fluorescence assay of a 15-bp dsDNA (500 µM) in the presence of peptoids at different ratios of peptoid amines to phosphate groups of the DNA (N/P) is shown. The fluorescence intensities of dsDNA/peptoid complexes at N/P of 4 (A and B) and dsDNA/PE2 complexes at different N/P (C and D) are plotted against the increasing temperature. In B and D, derivatives of fluorescence intensities representing the melting temperature (Tm) of dsDNA are plotted against the increasing temperature.