Fig. 8.
Surface functionalization of peptoid-stabilized OCs with antibody and fluorophore. (A) Schematic view showing alkyne-modified peptoids conjugates azide-modified cargos through click chemistry. PE8, Nae–Nprp–(Nae–Nte)10–Nae–Nprp; PE9, Nae6–Nte12–Nprp2, in which prp is the propargyl group. Here, Azide-Fluor 488 (AF) and Tz were used as the presenting cargos (label 1). (B) Chemical structure of PE8 and PE9. (C) Fluorescence assay of bare OCs, OC/PE8-AF, and OC/PE9-AF extracted from the agarose gel (λex = 485 nm and λem = 510 to 700 nm). (D) TEM images show surface coating of OCs with PE8-Tz and PE9-Tz. The samples were labeled with immunogold (label 2 in A) prior to TEM imaging. (Scale bars, 50 nm.)