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. Author manuscript; available in PMC: 2020 Aug 6.
Published in final edited form as: Nature. 2020 Feb 6;579(7800):598–602. doi: 10.1038/s41586-020-2013-6

Extended Data Figure 2. Analytical size-exclusion chromatography analysis of HPF1-PARP1 interaction.

Extended Data Figure 2

Uncropped SDS-PAGE gels with fractions from analytical size-exclusion chromatography. HPF1 was analysed in the presence or absence of PARP1 and either alone or with a short DNA duplex and/or the NAD+ analogue EB-47. Images from Fig. 1d are identical with areas marked with grey rectangles. Note the changed elution profile of PARP1 itself in the presence of DNA and EB-47, especially the shift in the peak centre on addition of DNA, possibly reflecting PARP1 oligomerisation.