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. Author manuscript; available in PMC: 2020 Aug 6.
Published in final edited form as: Nature. 2020 Feb 6;579(7800):598–602. doi: 10.1038/s41586-020-2013-6

Extended Data Figure 3. Analytical size-exclusion chromatography of HPF1-PARP1 CAT interaction.

Extended Data Figure 3

a, Uncropped gels with fractions from analytical size-exclusion chromatography shown in Fig. 1e.

b, Analytical size-exclusion chromatography analysis of PARP1 CAT binding to HPF1. PARP1 CAT was used with its lipoyl tag (see Materials and Methods) uncleaved to allow it to be distinguished from HPF1, which has approximately the same molecular weight and elution profile as PARP1 CAT (data not shown). For uncropped gels, see the HPF1 gel in a and two gels in c.

c, Uncropped gels for the analysis shown in b.

Contaminants of HPF1 are marked with *