Serum n-acetyl cysteine (NAC) concentrations and 4-point bend testing in ethanol (EtOH)-fed rats following treatment with NAC. EtOH-fed rats were injected with NAC at 200, 400, and 800 mg/kg body weight (b.w.) for 3 days. Serum and femur bones were collected and tested for (A) serum NAC concentrations determined by HPLC following 3 or 21 days of injections. (B) Four-point bend testing on femurs following EtOH and NAC administration. Significantly different compare to EtOH alone NAC 200 (***p < 0.001), NAC 400 (**p < 0.002), and NAC 800 (*p < 0.03), respectively. N = 6 for control and EtOH-fed rats. N = 3 for 200, 400, and 800 mg/kg b.w. NAC-treated EtOH-fed rats.