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. 2020 Mar 6;117(12):6752–6761. doi: 10.1073/pnas.1918859117

Fig. 3.

Fig. 3.

I-CeuI+ cultures were treated with the drug D-cycloserine to kill parent cells to yield a pure SimCell culture. (A) SimCell culture without glycolysis (Top) not induced and (Bottom) induced for GFP and mCherry production. (B) SimCell culture with glycolysis (Top) not induced and (Bottom) induced for GFP and mCherry production. No cells fluoresced green, which meant D-cycloserine was effective in killing parent cells and producing a pure SimCell culture. A higher proportion of SimCells (no GFP expression) expressed mCherry when supplemented with glycolysis (pSEVA224-GB3).