Table 2.

Purification of SimCells with D-cycloserine

Time (h)	ICeuI−glycolysis−	ICeuI+ glycolysis−	ICeuI− glycolysis+	ICeuI+ glycolysis+
0	1.8 × 109	3.5 × 105	1.0 × 109	3.08 × 105
1.5	1.1 × 109	4.1 × 103	6.9 × 108	2.7 × 103
5	1.3 × 107	7.2 × 102	4 × 107	7.5 × 102
24	1.6 × 107	0	8.3 × 104	0

Cultures were treated with 200 µg/mL D-cycloserine at time 0 and 1.5 h to kill normal cells to yield a pure SimCell culture. Plate counts over time estimated the CFU/mL and efficacy of the drug. Several dilutions were plated to ensure normal cells were killed. D-cycloserine effectively killed normal cells in strains with I-CeuI, indicating that it was an effective drug.