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. 2020 Mar 9;117(12):6509–6520. doi: 10.1073/pnas.1912375117

Fig. 6.

Fig. 6.

Down-regulation of target gene expression upon DBC1 knockdown is critically dependent on ELL. (A) The qRT-PCR analysis showing the effect of DBC1 knockdown, by using two different shRNAs, on basal level expression of target genes when compared to control scramble knockdown cells. (B) ChIP analysis showing the recruitment of target factors at the TSS region of indicated target genes upon DBC1 knockdown. (C) The qRT-PCR analysis showing the effect of reexpression of ELL on expression of selected target genes in DBC1 knockdown cells. (D) Cartoon diagram depicting mechanistic understanding of the role of DBC1, p300, HDAC3, and Siah1 in regulating ELL expression and downstream effect on expression of target genes. DBC1 knockdown results in simultaneous down-regulation of ELL by the action of HDAC3 deacetylase and Siah1-mediated polyubiquitylation. Reduced ELL level thus causes impaired expression of its target genes. In all of our statistical analyses, one-tailed Student’s t test was used to calculate the statistical significance of the data, wherein * denotes P ≤ 0.05, ** denotes P ≤ 0.01, *** denotes P ≤ 0.001, and ns denotes not significant.