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. Author manuscript; available in PMC: 2021 Mar 18.
Published in final edited form as: ACS Chem Neurosci. 2020 Mar 3;11(6):960–968. doi: 10.1021/acschemneuro.0c00008

Figure 4.

Figure 4.

[3H]Scopolamine competition binding at M1Rs reveals low micromolar affinity of α-PHP. Data are expressed as normalized means (±SEM) of three, independent determinations, with drugs tested at each concentration in triplicate. Curves for PSCs were best fit with a one-site model, whereas the oxotremorine curve best fit to a two-site model, suggestive of oxotremorine’s agonist activity, recognizing agonist high and low affinity M1R conformations. Note that α-PHP exhibited the highest affinity at M1Rs of all PSCs tested.