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. 2020 Mar 29;20:91. doi: 10.1186/s12903-020-1055-3

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© The Author(s). 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Expression of melatonin receptors MT1 and MT2 in normal and inflamed pulps. 3A and 3D show H&E staining of normal and inflamed pulp under 200X magnification level, respectively. 3B and 3C illustrate MT1 and MT2 staining in normal pulps, whereas 3E and 3F show MT1 and MT2 expression profiles in inflamed pulp. In normal healthy pulps, both MT1 and MT2 were intensely expressed in odontoblastic layer and cell-rich zones. Co-localization of MT1 and MT2 with pulpal fibroblasts was observed in pulpal stroma (arrow heads; 3B and 3C). In inflamed pulp, increased vascularization (arrows; 3D) and leukocyte infiltration (bold arrows; 3D) were seen in pulpal stroma. Similar to normal healthy pulps, MT1 and MT2 were found in odontoblastic layers (arrows; 3E and 3F) and co-localized with infiltrating leukocytes located in the pulp core (bold arrows; 3E and 3F)