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Characterization of MV functionality by turbidity and TNAP activity analyses. (A) Turbidity time course data from MV solutions with (MV+Ca2+) and without (MV Neat) Ca2+. SCL buffer with (SCL+Ca2+) and without Ca2+ (SCL) were used as negative controls. (B) TNAP activity assay of MVs before Ca2+ addition (MV Neat) and 200 min after addition (MV+Ca2+).
