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Activated myofibroblasts are localized to regions of scarring in TC‐treated mice. Immunofluorescence staining of 8‐µm sections of fixed, frozen kidneys from TC‐treated Gli1‐tdTomato mice 28 dpi show that activated myofibroblasts stain positively for αSMA+ (violet), Gli1+ (red), and PDGFRβ+ (green). Gomori trichrome staining of the adjacent section demonstrates that the activated myofibroblasts reside in areas of high collagen deposition and inflammation. Scale bar represents 50 µm
